Coding

Part:BBa_K137013:Design

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-06-20)


folC (folylpolyglutamate synthase + dihydrofolate synthase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 860
    Illegal EcoRI site found at 979
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 860
    Illegal EcoRI site found at 979
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 860
    Illegal EcoRI site found at 979
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 860
    Illegal EcoRI site found at 979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 860
    Illegal EcoRI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs.


Source

L.lactis genome

References